E and angina pectoris; primarily by way of inhibiting lipid oxidation, lowering cholesterol content in serum and antithrombotic effects, it may stop atherosclerosis, so as to treat coronary heart illness.[2,710] This paper adopts in vitro key cultured neonatal rat cardiomyocytes and types the hypoxia/reoxygenation injury model[1118] to simulate in vivo ischemiareperfusion injury, so as to study the effects of ferulic acid and its drug containing plasma on cardiomyocyte injury and to explore its mechanism, and its advantages are that it may exclude internal neurohumor along with the interaction involving local various varieties of cells to a really fantastic extent, and it might provide the basis for creating new drugs for coronary heart disease with clinical application worth.Key cultured neonatal rat cardiomyocyte cultivationWe take quite a few SD neonatal rats, take out the hearts beneath sterile situations, wash off residual blood with PBS, reduce the ventricles into 1m3 pieces, location them within a culture dish, add in suitable quantity of cold PBS, use trypsin and collagenase I mixture digestive fluid, repeat digestion in 37 water bath, gather cell suspension, separate and purify cardiomyocytes with differential wall adhesion process, place cardiomyocyte suspension in culture flask, culture in 37 and five CO2 incubator for 60 min, cardiac fibroblasts adhere towards the wall immediately, extract cell suspension, regulate cell concentration to 510 ?105 cells/ml with highglucose DMEM culture solution containing ten fetal calf serum, transfer to 96-pore plate to culture, get purer cardiomyocytes, culture in 37 and five CO2 situation, and select the cardiomyocytes with very good growth for the experiment right after 96 h.2356229-58-6 web Preparation of cardiomyocyte hypoxia/reoxygenation model[11-18]The effects of unique concentrations of sodium hydrosulfite on cardiomyocytesMATERIALS AND METHODSDrugs and reagentsFerulic acid (Batch No.: 07739910, purity higher than 98 , Chinese Biological Product Inspection Institute), MTT (GIBCO Company, USA), dimethyl sulfoxide (DMSO, Beijing Solarbio Science and Technology Co., Ltd.), trypsin (Difco Organization, USA), collagenase I (GIBCO Corporation, USA), DMEM highglucose and lowglucose culture option (GIBCO Organization, USA), sodium hydrosulfite (Tianjin Third Chemical Reagent Factory), verapamil hydrochloride tablets (Tianjin Central Pharmaceutical Co.BuyTaltobulin intermediate-1 , Ltd.), Yangxinshi tablets (Qingdao Guofeng Pharmaceutical Co., Ltd.), lactate dehydrogenase (LDH) and ATP assay kit(Nanjing Jiancheng Bio Co., Ltd), other reagents are commercial analytical pure.AnimalsTake principal cultured neonatal rat cardiomyocytes cultivated for four days and randomly divide into 7 groups, ten pores every group: standard control group: typical high glucose culture resolution; lowglucose culture resolution group; 800 M, 400 M, 200 M, one hundred M and 50 M concentrations of sodium hydrosulfite resolution + lowglucose culture remedy group; place in 37 and five CO2 incubator, and following culture for 1 h, replace with standard highglucose culture answer to continue.PMID:24324376 [13,15,16]The effects of various action time of sodium hydrosulfite on cardiomyocytesSD neonatal rats (1 three d) and Kunming mice, supplied by the Animal Center of Liaoning University of Traditional Chinese Medicine, reaching the national cleaning typical.Primary instrumentsTake primary cultured neonatal rat cardiomyocytes cultivated for four days and randomly divide into 7 groups, 10 pores every single group: standard manage group: normal highglucose culture solutio.